Journal article
In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics
ACG Weiss, K Krüger, QA Besford, M Schlenk, K Kempe, S Förster, F Caruso
ACS Applied Materials and Interfaces | AMER CHEMICAL SOC | Published : 2019
Abstract
In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evo..
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Grants
Awarded by Seventh Framework Programme
Funding Acknowledgements
This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio Nano Science and Technology (project no. CE140100036). K.K. and F.C. acknowledge the awards of a National Health and Medical Research Council (NHMRC) - ARC Dementia Research Development Fellowship (APP1109945) and an NHMRC Senior Principal Research Fellowship (APP1135806), respectively. A.C.G.W. acknowledges funding from The University of Melbourne through a Melbourne International Research Scholarship. This work was also supported by the European Research Council (ERC) Advanced Grant STREAM (project 291211). The authors also acknowledge the financial support by the German Academic Exchange Service (DAAD) through its Thematic network Bayreuth-Melbourne Colloid/Polymer Network sponsored from funds of the Federal Ministry of Education and Research (BMBF). We acknowledge F. Akgok, R Suckfull, and M. Faria for assistance with measurements.